■RESEARCH INTEREST
Marine bioactive lipids;Lipidomics; Seafood chemistry
My current research is mainly focused onmarine bioactive lipids, including preparation, analysis and characterization of complex lipids (e.g. cerebrosides, gangliosides, sulfatides, DHA/EPA-enriched phospholipids andplasmalogens); trying to understand the hydrolysis and oxidation mechanisms of phospholipids, glycerides and free fatty acids in the processing and storage of seafood; evaluation effects of bioactive lipids on Alzheimer’s disease and metabolic syndrome through Lipidomics strategy.
Research Experiences
My research interests have revolved around separating, analyzing, and identifying bioactive substances from seafood, especially rare and traditional seafood in China, such as sea cucumber, sea urchin, abalone, algae, etc. A variety of bioactive substances in seafood are identified with characteristic structures, for example fucoidans, collagens, phospholipids, sphingolipids, astaxanthins and so on. These compounds not only serve as nutrition, but also impact to human health. During the three-month period at Kyoto University, I focused on the field of cerebrosides from sea cucumber in Dr. Takashi Hirata’s lab.
Awards
ØOutstanding Teacher Award, Ocean University of China, 2015.
ØScientific Advancement Award, Shandong Province.2014 (Co-winner).
Ø“Tiantai Talents” Prize, Ocean University of China, 2014.
ØExcellent Award for Academic Paper, Analysis and Testing Academic Society of Qingdao City, 2011.
ØAdvanced Faculty Award, Ocean University of China, 2009.
ØExcellent Award for Academic Paper, Institute of Physical and Chemical Analysis and Testing of Shandong Province, 2009.
ØScientific Advancement Award, Ocean University of China, 1998 (Co-winner).
Research Grants
Principal Investigator:
1.The Natural Science Foundation of China (No. 31571864), The influence mechanisms of molecular structures and food matrix on the stability and bioavailability of astaxanthin esters. 2016-2019.
2.The Natural Science Foundation of China (No. 31201329), Structure analysis of sea cucumber cerebrosides and its protective effect on neurons in brain. 2013-2015.
3.The Specialized Fund for Basic Research Operating Expenses Program of Central College (No. 201251009),Study on the application of liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) in the analysis of sea cucumber glycolipids. 2012.1-2012.12.
4.The Transformation of Agricultural Scientific and Technological Achievements (No. 2009GB2C600195), Technology transformation and demonstration of high efficiency utilization of low-value aquatic products.2009-2011.
Co-Investigator:
1.The State Key Program of National Natural Science of China (No. 31330060), The changes of DHA/EPA phospholipid structure and nutrition in marine food processing and storage. 2014-2018.
2.The National Natural Science Foundation of China (No. 31371757), Regulatory effect and molecular mechanism of fish oil on the tertiary amine metabolism mediated metabolic syndrome. 2014-2017.
3.The 12th Five Year Program for Science and Technology Support (No. 2012BAD33B07), Key techniques of the preparation, activity evaluation and application of food ingredients regulating neural cell function.2012-2015.
4.The Special Fund for Agro-scientific Research in the Public Interest (No. 201105029),High efficiency preparation technology and industrialization demonstration of functional factors from seafood. 2011-2015.
5.The International Science & Technology Cooperation Program of China (No. 2010DFA31330), Study on the technology of water environment pollution, aquatic food safety and waste comprehensive utilization. 2010-2013.
6.The New Teacher Fund of Ministry of Education of China(No. 20100131120018), Study on the absorption and metabolism of the saponins from the sea cucumber Pearsonothria graeffei by LC-MS/MS.2010-2012.
■PUBLICATIONS
Peer-reviewed articles:(*Corresponding Author; 1Co-first Author)
1. Zicai Jia, Yu Song, Suyuan Tao, Peixu Cong, Xiaoxu Wang, Changhu Xu, Jie Xu*. Structure of sphingolipids from sea cucumber Cucumaria frondosa and structure-specific cytotoxicity against human HepG2 cells. Lipids, DOI: 10.1007/s11745-016-4128-y.
2. Yanjun Liu, Jie Xu*, Yong Xue, Xiaofang Liu, Jingfeng Wang and Changhu Xue*.Assessment of total and organic vanadium levels and their bioaccumulation in edible sea cucumbers: tissues distribution, inter species specific, locational differences and seasonal variations. Environmental Geochemistry and Health. 38(1): 111-122, 2016[PMID 25732906]
3. Yanjun Liu, Jie Xu*, Yongli Guo, Xin Zhang, Jingfeng Wang, Changhu Xue*. Green synthesis of oxovanadium (IV)/chitosan nanocomposites and its ameliorative effect on hyperglycemia, insulin resistance, and oxidative stress. Biological Trace Element Research. 169(2): 310-319, 2016[PMID 26144273]
4. Zicai Jia, Peixu Cong, Hongwei Zhang, Yu Song, Zhaojie Li, Jie Xu*, Changhu Xue. Reversed‑phase liquid chromatography–quadrupole‑time‑of‑flight mass spectrometry for high-throughput molecular profiling of sea cucumber cerebrosides.Lipids, 50(7): 667-679, 2015 [PMID26037520]
5. Zicai Jia, Shiyan Li, Peixu Cong, Yuming Wang, Tatsuya Sugawara, Changhu Xue, Jie Xu*. High throughput analysis of cerebrosides from the sea ucumber Pearsonothria graeffei by liquid chromatography—quadrupole-time-of-flight mass spectrometry. Journal of Oleo Science. 64(1): 51-60, 2015[PMID25492230]
6. Peixu Cong1, Ruichang Gao1, Changhu Xue, Zhaojie Li, Hongwei Zhang, Muhammad Naseem Khana, Yong Xue, Tatsuya Sugawara, Jie Xu*. Molecular species analysis of monosialogangliosides from sea urchin Strongylocentrotus nudus by RPLC-ESI-MS/MS. Food Chemistry. 166: 473–478, 2015[PMID25053082]
7. Shu Yang, Qingxin Zhou, Lu Yang, Yong Xue, Jie Xu*, Changhu Xue. Effect of thermal processing on astaxanthin and astaxanthin esters in pacific white shrimp Litopenaeus vannamei. Journal of Oleo Science. 64(3): 243-253,2015[PMID25757428]
8. Qingxin Zhou, Jie Xu, Shu Yang, Yong Xue, Ting Zhang, Jingfeng Wang, Changhu Xue*. The Effect of various antioxidants on the degradation of O/W microemulsions containing esterified astaxanthins from Haematococcus pluvialis. Journal of Oleo Science. 64(5): 515-525, 2015[PMID25843284]
9. Shuang Guo, Changhu Xue, Guoyun Li, Xue Zhao, Yuming Wang, Jie Xu*. Serum levels of glycosaminoglycans and chondroitin sulfate/hyaluronic acid disaccharides as diagnostic markers for liver diseases. Journal of Carbohydrate Chemistry. 34:55-59, 2015
10.Yanyan You,Yan Cao, Shuang Guo, Jie Xu*, Zhaojie Li, Jingfeng Wang, Changhu Xue. Purification and identification of α2-3 linked sialoglycoprotein and α2-6 linked sialoglycoprotein in edible bird’s nest. European Food Research and Technology. 240(2): 389-397, 2015
11. Yanjun Liu, Hui Xu, Jie Xu*, Yongli Guo, Yong Xue, Jingfeng Wang, Changhu Xue*. Vanadium-binding protein from vanadium-enriched sea cucumber Apostichopus japonicus inhibits adipocyte differentiation through activating WNT/β-catenin pathway. Journal of Functional Foods. 17: 504-513, 2015
12. Yanjun Liu, Jie Xu*, Yongli Guo, Yong Xue, Jingfeng Wang, Changhu Xue*. Ameliorative effect of vanadyl (IV)-–ascorbate complex on high-fat high-sucrose diet-induced hyperglycemia, insulin resistance, and oxidative stress in mice. Journal of Trace Elements in Medicine and Biology. 32: 155-161, 2015 [PMID 26302923]
13. Yanjun Liu,Jie Xu, Yong Xue, Jingfeng Wang, Yaoguang Chang, Changhu Xue*. Determination of trace vanadium in sea cucumbers by ultrasound-assisted cloud point extraction and graphite furnace atomic absorption spectrometry. International Journal of Environmental Analytical Chemistry. 95(3): 258-270, 2015
14. Hui Xu1, Jie Xu1, Yiming Wang, Shiwei Hu, Yuming Wang, Jingfeng Wang, Changhu Xue. Fucoidan isolated from the sea cucumber Acaudina molpadioides improves insulin resistance in adipocytes via activating PKB/GLUT4 pathway. European Food Research and Technology. 240(4): 753-761, 2015
15. Xiang Gao, Jie Xu, Chengzi Jiang, Yi Zhang, Yong Xue, Zhaojie Li, Jingfeng Wang, Changhu Xue*, Yuming Wang*. Fish oil ameliorates trimethylamine N-oxide exacerbated glucose intolerance in high-fat diet-fed mice. Food & Function. 6: 1117-1125,2015 [PMID 25686243]
16. Xiang Gao, Xiaofang Liu, Jie Xu, Changhu Xue, Yong Xue, Yuming Wang. Dietary trimethylamine N-oxide exacerbates impaired glucose tolerance in mice fed a high fat diet. Journal of Bioscience and Bioengineering. 18(4): 476-481, 2014[PMID 24721123]
17. Xiaofang Liu, Jie Cui, Zhaojie Li, Jie Xu, Jingfeng Wang, Changhu Xue, Yuming Wang*. Comparative study of DHA-enriched phospholipids and EPA-enriched phospholipids on metabolic disorders in diet-induced-obese C57BL/6J mice. European Journal of Lipid Science and Technology. 116(3): 255-265, 2014
18. Jie Xu, Shuang Guo, Lei Du, Yuming Wang, Tatsuya Sugawara, Takashi Hirata, Changhu Xue*. Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography. Journal of Oleo Science. 62(3):133-142, 2013[PMID23470440]
19. Fengjuan Wu, Yong Xue, Qingjuan Tang, Jie Xu, Lei Du, Changhu Xue, Koretaro Takahashi, Yuming Wang. The protective effects of cerebrosides from sea cucumber and starfish on the oxidative damage in PC12 cells. Journal of Oleo Science.62(9): 717-727, 2013 [PMID24005016]
20. Lei Du, Zhaojie Li,Jie Xu, Jingfeng Wang, Yong Xue, Changhu Xue, Koretaro Takahashi, Yuming Wang*. The anti-tumor activities of cerebrosides derived from sea cucumber Acaudina molpadioides and starfish Asterias amurensis in vitro and in vivo. Journal of Oleo Science. 61(6): 321-330, 2012 [PMID22687777]
21. Shuang Guo, Changhu Xue, Jie Xu*, Yuming Wang, Peixu Cong. Three glucocerebroside molecular species from the sea cucumber Apostichopus japonicus. Advanced Materials Research. 524-527: 2334-2339, 2012
22. Xiaoqian Hu1,Jie Xu1, Yong Xue, Zhaojie Li, Jingfeng Wang, Jiahui Wang, Changhu Xue, Yuming Wang*. Effects of bioactive components of sea cucumber on serum, liver lipid profile and lipid absorption.Bioscience, Biotechnology, and Biochemistry. 76(12): 2214-2218, 2012[PMID23221720]
23. Xiao Tang, Zhaojie Li, Jie Xu, Yong Xue, Jinzhang Li, Jingfeng Wang, Teruyoshi Yanagita, Changhu Xue, Yuming Wang*. Short term effects of different omega-3 fatty acid formulation on lipid metabolism in mice fed high or low fat diet. Lipids in Health and Disease. 11: 70, 2012 [PMID22676394]
24. Bei Zhang, Changhu Xue, Xiaoqian Hu, Jie Xu, Zhaojie Li, Jingfeng Wang, Teruyoshi Yanagita, Yong Xue*, Yuming Wang. Dietary sea cucumber cerebroside alleviates orotic acid-induced excess hepatic dipopexis in rats.Lipids in Health and Disease. 11: 48,2012 [PMID22569330]
25. Jie Xu, Jingjing Duan, Changhu Xue*, Tingyu Feng, Ping Dong, Tatsuya Sugawara, Takashi Hirata. Analysis and comparison of glucocerebroside species from three edible sea cucumbers using liquid chromatography–ion trap-time of flight mass spectrometry. Journal of Agricultural and Food Chemistry. 59(22):12246-12253, 2011 [PMID22004409]
26. Jie Xu, Yuming Wang, Tingyu Feng, Bei Zhang, Tatsuya Sugawara, Changhu Xue*. Isolation and anti-fatty liver activity of a novel cerebroside from the sea cucumber Acaudina molpadioides.Bioscience, Biotechnology, and Biochemistry. 75(8):1466-1471, 2011 [PMID21821952]
27. Qingjuan Tang, Dan Li, Jie Xu, Jingfeng Wang, Yuran Zhao, Zhaojie Li, Changhu Xue. Mechanism of inactivation of murine norovirus-1 by high pressure processing. International Journal of Food Microbiology. 137(2-3): 186-189,2010 [PMID20034691]
28. Qi Wang, Changhu Xue, Zhaojie Li, Jie Xu. Phosphatidylcholine levels and their fatty acid compositions in squid egg, a comparison study with pollack roe and sturgeon caviar. Journal of Food Lipids. 21(4): 356-359,2008
29. Shiguo Chen, Jie Xu, Changhu Xue, Ping Dong, Wenjing Sheng, Guangli Yu and Wengang Chai. Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid Ommastrephes bartrami by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy. Glycoconjugate Journal. 25: 481-492, 2008 [PMID18219573]
30. Qingxi Zhao, Jie Xu, Changhu Xue, Wenjing Sheng, Ruichang Gao, Yong Xue. Determination of biogenic amines in squid and white prawn by high-performance liquid chromatography with postcolumn derivatization. Journal of Agriculture and Food Chemistry. 55: 3083-3088,2007 [PMID17381105]
Book Chapters:
1.Editorial Board, Function Marine Biological Molecules—Discovery and Application, Chapter 14. Science Press, Beijing, China. 2007
2.Associate Editor, Marine Bioactive Substances. Publishing House of Ocean University of China, Qingdao, China. 2007
3.Editorial Board, Marine Health Food, Chapter 4. Chemical Industry Press, Beijing, China. 2009
