Xingguo Liang

Prof. Dr. Xingguo Liang graduated from College of Chemical Engineering, Tianjin University, for Bachelor (1988-1992) and Master (1992-1995). After working in Tianjin University as a lecturer, he got his Ph.D degree in 2001 from Department of Chemistry and Biotechnology, University of Tokyo, Japan. As a postdoc, he worked in Center for Advanced Science and Technology, University of Tokyo from 2001 to 2003, and in Department of Biomedical Engineering, Boston University from 2003 to 2005. From 2006-2011, he worked in the Department of Chemistry and Biotechnology and the Department of Molecular Design and Engineering, Nagoya University as an assistant/associate professor. Now, as a professor in OUC, his research interest is in the field of Nucleic Acids Chemistry, Food Science, Food Saftey, and Biotechnology. His group is working on the artificial regulation of DNA/RNA function, metabolism and detection of DNA/RNA, nanomaterial for food industry, etc. He published more than 70 papers on Angew. Chem. Int. Ed., J. Chem. Am. Soc., Small, Nano Lett., Nat. Protoc., Chem. Comm., Chem. Eur. J., Chem. Asia J., J. Mater. Chem.,Biochemistry and FEBS J. etc.

PUBLICATIONS

Ⅰ Articles (recent 3 years)

1)Dong, P.; An, R.; Jia, X.S.; Han, D.; Liang X.G.* Effects of different processing methods on oyster DNA. Lecture Notes in Electrical Engineering, 2014, 251, 1361-1372.

2)Wang, Y.; Dong, P.; Liang X.G.* Elongation of Trinucleotide Repeats by DNA Polymerase. Lecture Notes in Electrical Engineering. 2014, 251, 1383-1392.

3)Wang, X.Y.; Wang, X.L.; Dong, P.; Suzuki, M.; Asanuma, H. Liang, X.G.* Detection of DNA target with highly enhanced specificity by self-circularization rolling circle amplification. Lecture Notes in Electrical Engineering. 2014, 251, 1449-1458.

4)Pan, X.M.; Chen, W.K.; Jia, X.S.; Dong, P.; Liang, X.G.* Evaluation of a whole genome amplification method based on improved ligation-mediated PCR. Lecture Notes in Electrical Engineering. 2014, 251, 1333-1343.

5)Liang, X.G.*; Li, J. Biotechnology of Azobenzene-modified DNA and RNA. Lecture Notes in Electrical Engineering. 2014, 251, 1317-1331.

6)Liang X.; Zhou M.; Kato K.; Asanuma H. Photoswitch nucleic acid catalytic activity by regulating topological structure with a universal supraphotoswitch. ACS Synth Biol.2013, 2(4):194-202.

7)Kou, B.; Guo, X.; Xiao, S.J.*; Liang, X.G.* Highly Efficient Room-Temperature Photoresponsive DNA Tethering Azobenzene Through Backbone-Inserted Glycerol via Ether Bond. Small. 2013, 9(23), 3939–3943.

8)Wang, Y.; Jia, L.M.; Dong, P.; Liang X.G.* Expansion of trinucleotide repetitive sequences and its mechanism. Prog. Biochem. Biophy. 2013, 40, 345-355.

9)Liang, X. G.;* Mochizuki, T.; Fujii, T.; Kashida, H.; Asanuma, H.* Design of an artificial functional nanomaterial with high recognition ability. Natural Computing, 2012, 11, 231-238.

10)Ito, H.; Urushihara, M.; Liang, X. G.;* Asanuma, H.* Improvement of RNAi activity and strand-selectivity of RISC formation by modified siRNA involving intercalators near 5'-termini. ChemBioChem, 2012, 13, 311-315.

11)Asanuma, H.;* Osawa, T.; Kashida, H.; Fujii, T.; Liang, X. G.; Niwa, K.; Yoshida, Y.; Shimada, N.; Maruyama, A. Polycation-chaperoned in-stem molecular beacon system. Chem. Commun., 2012, 48, 1760-1762.

12)Nishioka, H.; Liang, X.G.;* Kato, T.; Asanuma, H.* Photon-fueled DNA nanodevice carrying two different photoswitches. Angew. Chem. Int. Ed., 2012, 51, 1165-1168.

13)Liang, X. G.;* Fujioka, K.; Asanuma, H.* Nick Sealing by T4 DNA Ligase on a Modified DNA Template Tethering a Functional Molecule on D-Threoninol. Chem. Eur. J., 2011, 17, 10388-10396.

14)Asanuma, H.; Nishioka, H.; Ishikawa, T.; Liang, X. G.; Preparation of photoresponsive DNA tethering ortho-methylated azobenzene as a supraphotoswitch. Current Protocols in Nucleic Acid Chemistry, 2011, 4.45.1-4.45.18. (DOI: 10.1002 / 0471142700. nc0445s46)

15)Liang, X.G.;* Mochizuki, T.; Fujii, T.; Kashida, H.; Asanuma, H. Design of a Functional Nanomaterial with Recognition Ability for Constructing Light-Driven Nanodevices. LNCS 2011, 6518, 112-122.

16)Asanuma, H.;* Toda, T.; Murayama, K.; Liang, X.G.; Kashida, H. Unexpectedly stable artificial duplex from flexible acyclic threoninol. J. Am. Chem. Soc., 2010, 132, 14702-14703.

17)Ito, H.; Liang, X.G.;* Nishioka, H.; Asanuma, H.* Construction of photoresponsive RNA for photoswitching RNA hybridization. Org. Biomol. Chem., 2010, 8, 5519-5524.

18)Tanaka, F.;* Mochizuki, T.; Liang, X.G.; Asanuma, H.; Tanaka, S.; Suzuki, K.; Kitamura, S.; Nishikawa, A.; Ui-Tei, K.; Hagiya, M. Robust and photo-controllable DNA capsules using azobenzenes. Nano Lett., 2010, 10, 3560-3565.

19)Hara, Y.; Fujii, T.; Kashida, H.; Sekiguchi, K.; Liang, X. G.; Niwa, K.; Takase, T.; Yoshida, Y.; Asanuma, H.* Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon.Angew. Chem. Int. Ed., 2010, 49, 5502-5506.

20)H. Kashida, K. Sekiguchi, X. G. Liang, H. Asanuma.* Accumulation of fluorophores into DNA duplexes to mimic the properties of quantum dots. J. Am. Chem. Soc., 2010, 132, 6223-6230.

21)Zhou, M.G.; Liang, X.G.*; Mochizuki, T.; Asanuma, H.* A light-driven DNA nanomachine for efficiently photoswitching RNA digestion. Angew. Chem. Int. Ed.2010, 49, 2167-2170. (Selected as a hot paper, reported by Chemistry World (Royal Society of Chemistry), Highlighted in Nature 2010, 464, 10.)

22)Liang, X.G.;* Wakuda, R.; Fujioka, K.; Asanuma, H. Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism. FEBS J., 2010, 277, 1551-1561. (selected as the front cover)

23)Nishioka, H.; Liang, X.G.;* Asanuma, H. * Effect of the ortho modification of azobenzene on the photoregulatory efficiency of DNA hybridization and thermal stability of its cis-form. Chem. Eur. J., 2010, 16, 2054-2062.

24)Liang, X.G.; Nishioka, H.; Mochizuki, T.; Asanuma, H. An interstrand-wedged duplex composed of alternating DNA base pairs and covalently attached intercalators. J. Mater. Chem.2010, 20, 575-581.

Ⅱ Books

1)Asanuma, H. Liang, X.G. Design of photoresponsive DNA by use of cartridge-type artificial nucleotide, in “New trend of nucleic acid chemistry”, 2011, the chemical society of Japan, Kagaku-Dojin Publishing Company, INC. ISBN: 9784759813661.

2)Liang, X.G.; Kato, T. Asanuma, H. De Novo DNA Synthesis by DNA Polymerase. in “Bacterial DNA, DNA Polymerase and DNA Helicases.” 2009, Nova Science Publishers, Inc. (English) ISBN: 9781607410942.

3)Asanuma, H.; Liang, X.G. The Dynamic Photoregulation of DNA. in “Science and Technology of Supramolecule – From Base to Innovation.” 2009, New Technology New Science Press. (Japanese) ISBN: 979-4-86043-309-3.

4)Zhou, C.L; Liang, X.G.; Diao, X.H.; Yang, H.M; Zhang, T.Y.; Wang, S.R. Experiment Methods for the Synthesis of Fine Chemicals. 1998, China Petrochemical Press. (Chinese) ISBN 7-80043-718-3. 4-43.

Ⅲ Invited Lectures (recent 3 years)

1)Australia Abnormal synthesis and amplification of DNA. 8th Asia-Pacific Conference on Algal Biotechnology and 1st International Conference on Coastal Biotechnology 2012, July 9-12, 2012, Adelaide, Australia.

2)Liang, X.G.; Zhou, M.G.; Nishioka, H.; Asanuma, H. Photoresponsive nucleic acids for constructing light-driven molecular machine as tools of biotechnology. Asian Congress on Biotechnology 2011, Nano- Analytical Biotech. D1107, May 15, 2011, Shanghai, China.

3)Liang X.G. Molecular biology and nanotechnology of azobenzene-modified nucleic acid, G-COE symposium in Nagoya on Biochemistry and Bionanotechnology. Jan. 12, 2011, Nagoya, Japan.

4)Asanuma, H.; Nishioka, H.; Mochizuki, T.; Liang, X.G. On-off photoswitching of DNA function with azobenzene-tethered DNA, Pacifichem 2010, Dec. 15-20, 2010, Hawai, USA.

5)Liang, X.G.; Mochizuki, T.; Nishioka, H.; Asanuma, H. Nanotechnology of azobenzene- modified DNA for constructing photoresponsive nanostructures and nanodevices (oral), PACIFICHEM 2010, December 15-20, 2010, Honolulu, USA.

6)Kato, T.; Liang, X.G.; Asanuma, H. Unexpected non-specific DNA synthesis by thermophilic DNA polymerase, PACIFICHEM 2010, December 15-20, 2010, Honolulu, USA.

7)Liang, X.G.; Fujioka, K.; Wakuda, R.; Asanuma, H. Reversible photoswitching of gene expression with azobenene-modified DNA, PACIFICHEM 2010, December 15-20, 2010, Honolulu, USA.

8)Itoh, H.; Liang, X.G.; Asanuma, H. Photoswitching of RNA function by using azobenzene- modified RNA, PACIFICHEM 2010, December 15-20, 2010, Honolulu, USA.

9)Liang, X.G.; Zhou, M.G.; Mochizuki, T.; Asanuma, H. X.G. Construction of Photoresponsive Nanodevices by Using a Novel Nanomaterial Involving Multiple Azobenzenes. The 37th International Symposium on Nucleic Acids Chemistry 2010, November 10-12, 2010, Yokohama, Japan.

10)Liang, X.G.; Kato, T.; Asanuma, H. DNA synthesis by thermophilic DNA polymerase in the absence of added DNA as template/primer. the 9th International Marine Biotechnology Conference, October 8-12, 2010, Qingdao, China.

11)Liang, X.G.; Mochizuki, T.; Fujii, T.; Kashida1, H.; Asanuma, H. Design of a Functional Nanomaterial with Recognition Ability for Constructing Light-Driven Nanodevices. DNA 16, June 14-17, 2010, Hong Kong, China.