Ping Dong

Associate Professor

Director of BPCS Training Center

College of Food Technology & Engineering, OceanUniversity of China,

Qingdao 266003, China

Telephone / Fax: 86-532-82031086, Mobile Phone: 13864223512

OBJECTIVE

Researches mainly includes: nutrition and food bioactivities; bioactive components chemistry(esp. isolation, purification& structure identification);chemical composition analysis &quality control(LC, GC, GC-MS, etc);nutrition evaluation; sea food processing &preservation; nucleic acids digestion and metablism.

EDUCATION

Ph.D.Sep 2003- Jun 2008,Aquatic Product Processing and Preserving Engineering, Ocean University of China,Qingdao, China.

Advisor: Prof. Chang-huXue.

Dissertation thesis: The nutrition evaluation of sea cucumbers and the study of bioactive components (esp. triterpene glycosides) in Pearsonothuriagraeffei.

B.A.Food Science and Engineering, Ocean University of China, Qingdao, China.

Sep 1999- Jun 2003.

PROFESSIONAL EXPERIENCE

Ocean University of ChinaJuly 2008 -Present

Associate Professor, College of Food Science and Engineering,from Jan. 2013

Assistant Professor, College of Food Science and Engineering

Researches mainly include:

(1)Nutrition, metabolismand bioactivities of sea cucumber.

(2) Food molecularbiology, especially, nucleic acid nutrition and metabolism.

Director of BPCS Training CenterSep 2011 - Present

Better Process Control School (BPCS) training, certifies supervisors of thermal processing systems, acidification, and container closure evaluation programs for low-acid and acidified foods (LACF/AF). These training courses must be approved by U. S. Food and Drug Administration (FDA).

The training center is offering the first approved BPCS training course in China.

University of MassachusettsSep 2008-Jul 2010

Post-doctoral Researcher, Department of Food Science

Advisor: Prof. Hang Xiao

Researches mainly include:

(1)Isolation, synthesis, and characterization of potential anti-carcinogenic dietary components from orange peel.

(2) Identification of key oncoproteins as targets of anti-carcinogenic components - polymethoxyflavones.

(3) Study on the cancer prevention bioactivities of polymethoxyflavones and combinations of flavonoid compounds withsome pharmacological drugs.

(4) The anti-cancer activities of triterpene glycosides from sea cucumber.

Ocean University of ChinaSep 2006-Sep 2008

Ph. D Student, College of Food Science and Engineering

Advisor: Prof. Chang-huXue

Researches mainly include:

(1)Preparation of structuremodified triterpene glycosides including the sapogenins and separation them by high-speed countercurrent chromatography (HSCCC) and silica gel.Study on the bioactivities of these holostanetriterpene glycosides with different chemical structures.

(2) Purification of holostanetriterpene glycosides by HSCCC.

(3) Primary studies on the figureprintand determinationofholostanetriterpene glycosides.

(4) Isolation and purification of sphingolipids from sea cucumber.

(5) Primary study onfucoxanthinwhich was a major carotenoid exists in phaeophyta. Especiallythe isolation&purification of fucoxanthinusing silica column, and chemical analysis of fucoxanthinby TLC, HPLC, ESIMS,etc.

Second Military MedicalUniversitySep 2005-Sep 2006

Ph. D Student, ResearchCenter for Marine Drugs, School of Pharmacy

Advisor: Prof. Yanghua Yi

Researches mainly include:

Isolation, purification of the triterpene glycosides in sea cucumber Pearsonothuriagraeffei by silica gel, RP-18 silica gel, sephadex LH-20 column chromatography and preparative HPLC, etc. Identified their chemical structures by IR, ESIMS and NMR(including ¹H, ¹³C, DEPT, HMQC, COSY, HMBC, TOCSY, NOESY, etc) analysis.

OceanUniversity of ChinaSep 2003-Sep 2005

Ph. D Student, College of Food Science and Engineering

Advisor: Prof. ChanghuXue

Researches mainly include:

(1) Chemical composition and bioactive compounds (including collagen, glyucosaminoglycan, triterpene glycosides, trace elements, etc)analysis ofvarious sea cucumbers.

(2) Nutrition evaluation of sea cucumbers usinganimal experimental models.

(3) Processing and preservation of various sea cucumber products (including dried, water-risen, instant and enzyme hydrolysisproducts, etc).

OceanUniversity of ChinaSep 1999-Jun 2003

Bachelor Student, College of Food Science and Engineering

Advisor: Prof. ChanghuXue

Researches mainly include:

Study on the H₂O₂ hydrolysis condition of fucoidanisolated fromLaminaria japonicaand preparation of the antioxidative low molecular weight fucoidan.

TRAINING PROGRAMS

1.Food Defense Collaborative Exchange Program. Conducted by the University of Minnesota’s National Center for Food Protection and Defense (UMN-NCFPD). St. Paul, Minnesota, US. June 4-8, 2012. (Invited and fully supported by USDA and FDA as representative from academia in China).

PUBLICATIONS

Articles

1.Jinkai Zheng, Mingyue Song, Ping Dong, PeijuQiu, ShanshanGuo, ZhimeiZhong, Shiming Li, Chi-Tang Ho, Hang Xiao*. (2013). Identification of novel bioactive metabolites of 5-demethylnobiletin in mice. Molecular Nutrition & Food Research. 57(11): 1999-2007.

2.NoppawatCharoensinphon, PeijuQiu, Ping Dong, Jinkai Zheng, PearlineNgauv, Yong Cao, Shiming Li, Chi-Tang Ho, Hang Xiao*. (2013). 5-Demethyltangeretin inhibits human nonsmall cell lung cancer cell growth by inducing G2/M cell cycle arrest and apoptosis.Molecular Nutrition & Food Research. 57(12): 2103-2111.

3.Yang Wang, LeiminJia,Ping Dong,Xingguo Liang.* (2013). Expansion of trinucleotide repetitive sequences and its mechanism.Prog. Biochem. Biophy. 40: 345-355.(Front Page, SCI 0.236).

王阳，贾蕾敏，董平，梁兴国. (2013).三核苷酸双链重复序列扩展合成特性及其机理.生物化学与生物物理进展.40: 345-355.（封面文章，SCI 0.236）.

4.Yanfang Zhang, Ping Dong*, Xingguo Liang. (2013). Advances in in vitro digestion models for edible substances. Sci. Technol. Food Ind. 34(18): 356-361.

张艳芳，董平*，梁兴国. (2013).食物成分消化模型的研究进展 [J].食品工业科技.4(18): 356-361.

5.Ping Dong, Ran An, XiushuangJia, Dong Han, Xingguo Liang. (2014). Effects of Different Processing Methods on Oyster DNA. Lecture Notes in Electrical Engineering. 251: 1361-1372.(EI).

6.Yang Wang, Ping Dong, Xingguo Liang. (2014). Elongation of Trinucleotide Repeats by DNA Polymerase. Lecture Notes in Electrical Engineering. 251: 1383-1392.(EI).

7.Xingyu Wang, Xiaoliang Wang, Ping Dong, Masatomo Suzuki, Hiroyuki Asanuma, Xingguo Liang.(2014). Detection of DNA Target with Highly Enhanced Specificity by Self-Circularization Rolling Circle Amplification. Lecture Notes in Electrical Engineering.251: 1449-1458 (EI).

8.Xiaoming Pan, Weikai Chen, XiushuangJia, Ping Dong, Xingguo Liang. (2014). Evaluation of a whole genome amplification method based on improved ligation-mediated PCR.Lecture Notes in Electrical Engineering. 251: 1333-1343.(EI).

9.Zhao Q, Xue Y, Wang JF, Li H, Long TT, Li Z, Wang YM, Dong P, Xue CH. (2012). In vitro and in vivo anti-tumour activities of echinoside A and ds-echinoside A from Pearsonothuriagraeffei.J Sci Food Agric. 92(4):965-74.

10.Xiaoliang Wang, Xingyu Wang, Changcheng Liang, Ping Dong, Xingguo Liang. (2012). Rolling circle amplification of DNA —simple and effective isothermal amplification technology of nucleic acid. Science and Technology of Food Industry. 16: 358-363.

11.ShanshanGuo, PeijuQiu, GuangXu, Xian Wu, Ping Dong, GuanpinYang,JinkaiZheng, David Julian McClements, Hang Xiao. (2012) Synergistic anti-inflammatory effects of nobiletin and sulforaphane inlipopolysaccharide-stimulated RAW 264.7 cells. J. Agric. Food Chem. 7;60(9):2157-64.

12.PeijuQiu, Huashi Guan, Ping Dong, Shiming Li, Chi-Tang Ho, Min-Hsiung Pan, David Julian McClements, Hang Xiao. (2011). The p53-, Bax- and p21-dependent inhibition of colon cancer cell growth by 5-hydroxy polymethoxyflavones. Molecular nutrition food research. 55(4): 613-622.

13.WasamonNutakul, Hana Shatara Sobers, PeijuQiu, Ping Dong, Eric Andrew Decker, David Julian McClements, Hang Xiao. Inhibitory effects of resveratrol and pterostilbene on human coloncancer cells: aside-by-side comparison. (2011). J. Agric. Food Chem. 59(20): 10964-10970.

14.PeijuQiu, Huashi Guan, Ping Dong, Shiming Li, Chi-Tang Ho, Min-Hsiung Pan, David JulianMcClements and Hang Xiao. (2011). The inhibitory effect of 5-hydroxy-3,6,7,8,3’,4’ -hexamethoxyflavone on human colon cancer cells. Molecular nutrition food research. 55: 1-10.

15.Xu J, Duan J, Xue C, Feng T, Dong P, Sugawara T, Hirata T.(2011). Analysis and comparison of glucocerebroside species from three edible sea cucumbers using liquid chromatography-ion trap-time-of-flight mass spectrometry.J. Agric. Food Chem. 59(22):12246-12253.

16.Qin Zhao, Zhi-dong Liu, Yong Xue, Jing-feng Wang, Hui Li, Qing-juan Tang, Yu-ming Wang, Ping Dong, and Chang-huXue. (2011). Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions. J. Zhejiang Univ. Sci. B. 12(7): 534-544.

17.Ping Dong, PeijuQiu, Yi Zhu, Shiming Li, Chi-Tang Ho, David Julian McClements and Hang Xiao. (2010). Simultaneous determination of four 5-hydroxy polymethoxyflavones by reversed-phase high performance liquid chromatography with electrochemical detection.J. Chromatogr. A. 1217(5): 642-647.

18.Qiu P, Ping Dong, Guan H, Li S, Ho CT, Pan MH, McClements DJ, Xiao H. (2010). Inhibitory effects of 5-hydroxy polymethoxyflavones on colon cancer cells. Molecular Nutrition & Food Research. 54(suppl 2): 244-252.

19.PeijuQiu, Ping Dong, Ryan Riddle and Hang Xiao. (2010). Synergistic inhibition of colon cancer cell growth by 5-hydroxy nobiletin and atorvastatin. FASEB J. 24 (Meeting Abstract Supplement) 928.13.

20.ChanghuXue, Lingfang Yu, Ping Dong, JieXu, ZhaojieLi, &Yong Xue. (2010). Isolation andidentification of three triterpene glycosides from sea cucumber ApostichopusjaponicusSelenka of Qingdao. Periodical of Ocean University of China, 40: 60–65.

21.Linfang Yu, Ping Dong, ChanghuXue, Yuming Wang, Jie Xu, Zhaojie Li, Yong Xue. (2010).High performance liquid chromatographic fingerprints of triterpene glycosides from Stichopusjaponicas.28(09): 885-888.

22.PeijuQiu, Huashi Guan, Ping Dong, Shiming Li, Chi-Tang Ho, Min-Hsiung Pan, David Julian McClements and Hang Xiao. (2010). Hydroxylatedpolymethoxyflavones induce p53 and Bax dependent apoptosis and cell cycle arrest.FASEB J.24 (Meeting Abstract Supplement) lb484.

23.NoppawatCharoensinphon, PeijuQiu, Ping Dong, PearlineNgauv and Hang Xiao. (2010). Inhibition of Lung Cancer Cell Growth by Polymethoxyflavones from Sweet Orange. FASEB J.24 (Meeting Abstract Supplement) 217.8.

24.WasamonNutakul, Hana Shatara Sober, PeijuQiu, Ping Dong and Hang Xiao. (2010). Inhibitory effects of resveratrol and pterostilbene on human colon cancer cells: a side-by side comparison.FASEB J.24 (Meeting Abstract Supplement) 928.14.

25.Qin Zhao, Yong Xue,Zhi-dong Liu, Hui Li, Jing-feng Wang, Zhao-jie Li, Yu-ming Wang, Ping Dong, Chang-huXue. (2010). Differential Effects of Sulfated Triterpene Glycosides, Holothurin A1, and 24-Dehydroechinoside A, on Antimetastasic Activity via Regulation of the MMP-9 Signal Pathway. J. Food Sci. 75(9): 280-288.

26.Ping Dong, PeijuQiu, ChanghuXue, Huashi Guan, Hang Xiao. (2009).Holothurin A, a sea cucumber triterpene glycoside inhibits the growth of colon cancer cells. The InternationalSymposium on Seafood Processing Technology and Safety Control System.

27.Ping Dong, Chang-huXue and Qi-zhen Du. (2008). Separation of two main triterpene glycosides from sea cucumber Pearsonothuriagraeffei by high-speed counter current chromatography (HSCCC). ActaChromatographica. 20(2): 269-276.

28.Ping Dong, Chang-huXue, Lin-fang Yu, Jie Xu and Shi-guo Chen. (2008). Determination of triterpene glycosides in sea cucumber (Stichopusjaponicus) and its related products by high performance liquid chromatography.J. Agric. Food Chem. 56(13): 4937-4942.

29.Shiguo Chen, Jie Xu, ChanghuXue, Ping Dong, Wenjing Sheng, Guangli Yu and Wengang Chai. (2008). Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid Ommastrephesbartrami by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy.Glycoconjugate Journal. 25(5): 481-492.

30.Ping Dong, Chang-huXue, Wen-jing Sheng, Jie Xu, Zhao-jie Li. (2008). Study on the determination of total triterpene glycosides in sea cucumbers. Chinese Journal of Marine Drugs, 27(1): 28-32. (in Chinese)

31.Feng-xia Cui, Chang-huXue, Zhao-jie Li, Yong-qin Zhang, Ping Dong, Xue-yan Fu and Xin Gao. (2007). Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopusjaponicus.Food Chemistry. 100(3):1120-1125.

32.Ping Dong, Chang-huXue, Zhao-jie Li, Jie Xu, Xue Zhao, Yue-piaoCai and Yong-qin Zhang. (2005). Preparation and antioxidative activity of low molecular weight fucoidan.Periodical of Ocean University of China, 36(sup.): 59-62. (in Chinese)

33.Min Lei, Jingfeng Wang, Zhaojie Li, Long Pang, Ping Dong, ChanghuXue. (2007). Effects of Sepia Product on Hemapoiecsis Promotion in ^60 Coγ Irradiated Mice. Food Science.2007, 2: 293-297. (In Chinese)

34.Long Pang, Jingfeng Wang, Ping Dong, Yi Wang, ChanghuXue. (2006).ActaNutrimentaSinica. 28(5): 446-447.

Patents

Ping Dong. A determination method of triterpene glycosides in sea cucumber.200810013642.X

Book Chapters

1.Yibin Zhou, Dongfeng Wang, Ping Dong. Chapter 6Victamins. Food Chemistry. 2012, Nova Science Publishers, Inc. New York. (English) ISBN 978-1-61942-125-7.

2.Zhao-jie Li, Yong Xue, Ping Dong, etal. Aquatic products chemistry, Chapter 9, 30,000 / 200,000 words. (2007). Published Chemical Industry Press.

Abstracts and Meeting presentation:

1.Ping Dong, Ran An, XiushuangJia, Dong Han, Xingguo Liang. (2012). Effects of Different Processing Methods on Oyster DNA. 2012 International Conference on Applied Biotechnology. Tianjin, China, Oct. 17-Oct. 19, 2012.

2.Ping Dong, PeijuQiu, ChanghuXue, Huashi Guan, Hang Xiao. (2009). Holothurin A, a sea cucumber triterpene glycoside inhibits the growth of colon cancer cells. The International Symposium on Seafood Processing Technology and Safety Control System. Qingdao, Shandong, China, Oct. 31-Nov. 2, 2009. (invited)

3.Ping Dong, Yang-hua Yi, Jing-feng Wang, Chang-huXue. (2007). The cytotoxic triterpene glycosides from sea cucumber Pearsonothuriagraeffei. The Fourth Chinese Food Graduate-Student Forum. Nanchang, Jiangxi, China.

4.Ping Dong, Chang-huXue, Jie Xu, Wen-jing Sheng. (2005). A method for polysaccharides determination in the body wall of sea cucumbers. International Conference Food Science and Technology VI. Guangzhou, Guangdong, China.

RESEARCH GRANTS

1. Study on Digestion and Uptake of Tolerant Nucleic Acid Based on in vitro Model. National Natural Science Foundation of China (NSFC, No. 31201327).2013.1-2015.12. Principle Investigator.260,000 RMB.

2. Study of the Uptake and MetabolismMechanismof Triterpene Glycosides from Pearsonothuriagraeffeiby LC-MS-MS. Doctoral Fund of Ministry of Education of China (RFDP, No. 20100132120018). 2011.1-2013.12.Principle Investigator.36, 000 RMB.

3. Study of the inhibition of colon cancer by bioactive compounds from sea cucumber. “Project of Cooperation and Training of High-Level Researchers with North America”supported byMinistry ofEducation of China. 2011.2-2012.2. Principle Investigator.100,000 RMB.

4. Study of the metabolism and anticancer activities of triterpene glycosides from sea cucumber. “Scientific Research Foundation for Returned Scholars, Ministry of Education of China”.2013.1-2014.12. Principle Investigator.30,000 RMB.

AWARDS AND HONORS

1.Creation & Invention Scholarship of Ocean University of China in 2004.

2.Outstanding graduates of Ocean University of China. (2003).

3.Excellent faculty of Ocean University of China. (2012).

PUBLIC SERVICES

1.Co-organized and serviced on“The 1stSymposium on Effective Utilization of Marine Bio-resources”. Qingdao, Shandong, China. May 24-26, 2013.

2.Co-organized and serviced on“International Symposium on Food Processing and Biotechnology 2012”. Qingdao, Shandong, China. May 23-25, 2012.

3.Serviced on “The 1st National College Students Aquatic Products Processing and Creative Competition”. Qingdao, Shandong, China. Aug 14-17, 2012.

PEER REVIEW

1.Grant Reviews

(1)National Natural Science Foundation of China (NSFC) 2012.12-

2.Journal Article Reviews

(1)Journal of Agriculture and Food Chemistry

(2)Journal of Food Processing and Preservation

(3)Food Science & Nutrition

(4)LWT - Food Science and Technology

COURSES

1.Food Biotechnology Experiments2011-2012

2.Genetic Engineering2011

3.Genetic Engineering Experiments2013-present

4.Experiments of Marine Bioactive Substances Isolation Technologies2014-present