Guoyun Li

Guoyun Li

Ph.D.

Professor

Doctoral Supervisor

Department

Laboratory of Glycochemistry & Glycotechnology

Phone

86-532-82031615

Email

liguoyun@ouc.edu.cn

Address

School of  Medicine and Pharmacy, Room A105, No. 5 Yushan  Road, Qingdao, Shandong, 266003

Research Area

1.    Glycomics:  isolation and purification, sequence, characterization, quantification and  high sensitive analysis.

2.    Quality Control and pharmacokinetic  studies of carbohydrate-based pharmaceutics.

3.    Research and  development of carbohydrate-based pharmaceutics

Biography

Dr. Guoyun  Li was born in August 1987. She completed her bachelor’s degree in Food  Science and Engineering from Ocean University of China (OUC) in 2009, and  obtained her Ph.D. degree under the guidance of Prof. ChanghuXue and Prof. Robert J. Linhardt  from OUC in 2015. During 2012-2014, Dr. Li was engaged in glycosaminoglycomics  research in the Linhardt’s laboratory in Rensselaer  Polytechnic Institute. In July 2015, Dr. Li joined in Prof. Guangli Yu’s group in School of Medicine and Pharmacy at OUC. Her current  research interest focuses on structure and functions of carbohydrates.

Education

2009.09~2015.06

Ocean University of China

Aquatic Products Processing and Utilization

Ph.D.

2012.09~2014.09

Rensselaer Polytechnic Institute

Glycochemistry

Co-educated Ph.D. student

2005.09~2009.06

Ocean University of China

Food Science and Engineering

B.S.

Work Experience

2020.12~Present

School of Medicine and Pharmacy, Ocean  University of China

Professor

2015.07~2020.11

School of Medicine and Pharmacy, Ocean  University of China

Associate Professor

2015.07~2018.01

School of Medicine and  Pharmacy, Ocean University of China

Postdoctoral Fellow

Academic Acitivities

Member  of American Chemical Society

Member  of American Society for Mass Spectrometry

Senior  member of Chinese Pharmaceutical Association

Honors

Courses

Glycan-based  pharmaceutics (for undergraduate)

Glycoengineering  and glycan-based drugs (for graduate)

Research Interests

Our research interests are centered on understanding  the structure and functions of carbohydrates. We prepare the polysaccharides  and their derived oligosaccharides from marine organism based  on feasible strategies to construct the marine carbohydrate database, and  develop novel analytical approaches to decode their fine complex structure  using sophisticated NMR and biomass spectrometry combined  glycobioinformatics. Based on above structural information and combined  computer simulation, carbohydrate microarray, and cell or animal-level  bioactivities screening methods, the structure–activity relationships of  marine polysaccharides try to be illustrated to discover interesting marine  bioactive carbohydrate. Besides, we also devote to resolve the critical pharmacokinetic  study of carbohydrate-based drugs, to elucidate the mechanisms of their  absorption, metabolism, transport and excretion. These studies will lay the  foundation for the research and development of marine carbohydrate-based  drugs.  

Another research direction in our lab focuses on  elucidating the structure and physiologicalfunction of endogenous  glycans (N-glycan, O-glycan, glycosaminoglycans and glycanlipids)  in human. Our research has focused on the development of high-throughput  technologies to isolate and identify the glycome  related to human health and disease, especially the profile of glycome in the clinical samples. Based on the  comprehensively evaluation of the glycome in the  different progress of critical illness, we attempt to elucidate the variation  of glycans and find the specific glycan. These findings could allow the  identification of relevant glycan biomarkers for the diagnosis, early  detection, and monitoring of cancer treatment with sufficient sensitivity and  specificity for clinical use, which could also enhance understanding of  critical illness.

Publications

Papers

1.Lin Pan, Xuze  Ai, Tianyu Fu, Li Ren, Qingsen  Shang*, Guoyun Li*, Guangli  Yu*. In vitro fermentation of hyaluronan by human gut microbiota:Changes in microbiota community and  potential degradation mechanism. , CarbohydratePolymers, 2021, 269, 118313

2.Ning Zhang, Guoyun Li*, Shijie  Li, Chao Cai, Fuming Zhang, Robert J. Linhardt, Guangli Yu.   Mass spectrometric evidence for the mechanism of free-radical  depolymerization of various types of glycosaminoglycans, Carbohydrate Polymers, 2020,  233: 115847

3.QingfengNiu, Guoyun Li*, Chao Li, Qinying Li, Jia Li, Chanjuan  Liu, Lin Pan, Shijie Li, Chao Cai, JieJie Hao, Guangli Yu. Two  different fucosylated chondroitin sulfates:  structural elucidation, stimulating hematopoiesis and immune-enhancing  effects. CarbohydratePolymers, 2020, 230: 115698

4.Chao Li, QingfengNiu, Shijie Li , Xin Zhang , Chanjuan  Liu , Chao Cai , Guoyun Li*, Guangli  Yu. Fucoidan from Sea Cucumber Holothuriapolii: Structural Elucidation and Stimulation of  Hematopoietic Activity.International Journal of Biological  Macromolecules, 2020,  154:1123-1131. 

5.Qinying Li, Mei Sun, Mingsheng Yu, Qianyun Fu, Hao Jiang, Guangli  Yu, Guoyun Li*. Gangliosides profiling  in serum of breast cancer patient: GM3 as a potential diagnostic biomarker. GlycoconjugateJournal, 2019, 36(5): 419-428.

6.Chanjuan Liu, Chao Li, Guoyun  Li*, Guangli Yu*. Carbohydrate  microarray-based analysis of specific interactions between saccharides from  algin and influenza A viral hemagglutinin. Analytical Methods.2019,  11, 3641–3647

7.Qianyun Fu, Guoyun  Li*, Chen Wang, Ya Wang, Qinying Li, Jiejie Hao and Guangli Yu. Profiling and Structural Characterization of  High Neu5Gc or Sulfate-Containing O-glycans from Hyla  Rabbit Intestinal Mucin. Molecules2019, 24, 1365.

8.Chanjuan Liu, Chao Li, QingfengNiu, Chao Cai, Guoyun  Li*, Guangli Yu*. Fabrication of  carbohydrate microarrays on poly(2-hydroxyethyl  methacrylate)-cyanuric chloride-modified substrates for the analysis of  carbohydrate–lectin interactions. NewJournal of Chemistry, 2019,43, 9145 - 9151

9.Qinying Li,Guoyun Li*, Yu Zhou, Xin Zhang, Mei Sun, Hao  Jiang, and Guangli Yu. Comprehensive N-Glycome Profiling of Cells and Tissues for Breast Cancer  Diagnosis. Journal of Proteome Research2019 18 (6), 2559-2570

10.   Xiaojiang Wang, Xiaoliang Zhao, YoujingLv, Minghua  Hu, Luodi Fan, Qinying  Li, Chao Cai, Guoyun Li*,  Guangli Yu*.  Extraction, isolation and structural characterization of a novel  polysaccharide from Cyclocaryapaliurus,  International Journal of Biological  Macromolecules, 2019, 132:  864-870,

11.   Hong Qiu, Songshan Shi, Jingwen Yue, Meng Xin, Alison V. Nairn,  Lei Lin, Xinyue Liu, Guoyun Li, Stephanie A.  Archer-Hartmann, Mitche Dela Rosa, Melina Galizzi, Shunchun Wang, Fuming  Zhang, Parastoo Azadi, ToinH.vanKuppevelt,  Wellington V. Cardoso, Koji Kimata, Xingbin Ai, Kelley W. Moremen,  Jeffrey D. Esko, Robert J. Linhardt and Lianchun Wang*. A mutant-cell library for systematic  analysis of heparan sulfate structure–function relationships, NatureMethods, 2018, 15(11),  889-899

12.   Kaiyun Cui, Wenjing Tai, Xindi  Shan, Jiejie Hao, Guoyun Li*, Guangli  Yu*. Structural characterization and anti-thrombotic properties of fucoidan  from Nemacystusdecipiens.  Internationaljournal of biological macromolecules, 2018, 120, 1817-1822.

13.   Chen Wang, Yinzhi Lang, Qinying Li, Xin Jin, Guoyun  Li*, Guangli Yu*. Glycosaminoglycanomic  profiling of human milk in different stages of lactation by liquid  chromatography-tandem mass spectrometry. FoodChemistry, 2018, 258,  231-236

14.   Qinying Li, Guoyun  Li*, Xiaoliang Zhao, Xindi  Shan, Chao Cai, Jing Zhao, Fuming Zhang, Robert J. Linhardt  and Guangli Yu,* Structural Characterization and  Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark  (Prionace glauca) Cartilage. MarineDrugs,  2018, 16(4), 128

15.   Qinying Li, Chao Cai, Yaoguang  Chang, Fuming Zhang, Robert J. Linhardt, ChanghuXue, Guoyun Li*, Guangli  Yu*, A novel structural fucosylated chondroitin  sulfate from Holothuriamexicana  and its effects on growth factors binding and anticoagulation, CarbohydratePolymers, 2018, 181:1160-1168

16.   Guoyun Li, Lingyun  Li, Eun Ji Joo, Ji Woong Son, Young Jin Kim, Jae  Ku Kang, Kyung Bok Lee, Fuming Zhang*,  Robert J. Linhardt*. Glycosaminoglycans and glycolipids as potential biomarkers in  lung cancer. GlycoconjugateJournal,  2017, 34(5), 661-669.

17.   Qingsen Shang, Qinying Li, Meifang Zhang, Guanrui Song, Jingjing Shi, Hao Jiang, Chao Cai, Jiejie  Hao, Guoyun Li*, and Guangli  Yu*. Dietary Keratan Sulfate from Shark Cartilage Modulates Gut Microbiota  and Increases the Abundance of Lactobacillus spp. MarineDrugs, 2016,  14(12), 224

18.   Xiao Zhang, Xiaoliang Zhao, Yinzhi Lang, Qinying Li, Xiaoxiao Liu, Chao  Cai, Jiejie Hao, Guoyun Li*, Guangli Yu*. Low Anticoagulant Heparin Oligosaccharides  as Inhibitors of BACE-1, the Alzheimer’s β-Secretase. CarbohydratePolymers, 2016, 151: 51-59

19.   Guoyun Li, Lingyun  Li, ChanghuXue, Dustin  Middleton, Robert J. Linhardt, Fikri  Y. Avci*, Profiling pneumococcal type 3-derived  oligosaccharides by high resolution liquid chromatograph-tandem massspectrometry, Journal of Chromatography A, 2015, 1397: 43-51。

20.   Guoyun Li^#, Lingyun Li^#,  Fang Tian, Linxia Zhang, ChanghuXue, Robert J. Linhardt*,  Glycosaminoglycanomics of cultured cells using a  rapid and sensitive LC-MS/MS approach, ACSChemicalBiology,2015,10(5):1303-1310。

21.   Guoyun Li, Bo Yang, Lingyun  Li, Fuming Zhang, ChanghuXue,  Robert J. Linhardt*, Analysis of 3-O-sulfo group-containing  heparin tetrasaccharides in heparin by liquid  chromatography–mass spectrometry, AnalyticalBiochemistry, 2014, 455: 3-9

22.   Guoyun Li, Julia Steppich,  Zhenyu Wang, Yi Sun, ChanghuXue, Robert J. Linhardt, Lingyun Li*, Bottom-up low molecular weight heparin  analysis using LC-FTMS for extensive characterization, AnalyticalChemistry,2014,86(13): 6626-6632

23.   Guoyun Li, Chao Cai, Lingyun  Li, Li Fu, Yuqing Chang, Fuming Zhang, Toshihiko Toida, ChanghuXue, Robert J. Linhardt*,  Method to detect contaminants in heparin using radical depolymerization and  liquid chromatography-mass spectrometry, AnalyticalChemistry, 2014, 86(1): 326-333

Books

Patents

Projects

1. 2020/01-2023/12, NSFC, 31971210, Structure-activity  relationship and mechanism studies of series of FCS oligosaccharides for  VEGF/FGF2-based inhibition of tumor angiogenesis, Project leader

2. 2017/01-2019/12, NSFC,  31600646, Establishment of GAGs expression database and biomarker validation  in the serum of breast cancer patients based on MS Multiple Reaction  Monitoring, Project leader