Laboratory of Marine Natural Product Biosynthesis
Principal Investigator
Prof. Dr. Wenli LI
School of Medicine and Pharmacy
Ocean University of China,
5 Yushan Rd.
Qingdao, Shandong266003, CHINA
Tel/Fax: (0532) 82031813
Email: liwenli@ouc.edu.cn
Dr. Wenli LI currently is Professor of Pharmaceutical Sciences in the School of Medicine and Pharmacy, Ocean University of China. She received her Ph.D. from the Institute of Microbiology, Chinese Academy of Sciences in 2003 (1998-2003), and carried out postdoctoral research in School of Pharmacy, University of Wisconsin-Madison (2004-2008). After a research career in the pharmaceutical product development (PPD) company in the US (2008-2009), she took a position as a full Professor in the School of Medicine and Pharmacy, Ocean University of China in 2009. Prof. Li’s current research focuses on the biosynthesis of secondary metabolitesfrommarine microorganisms, and metabolic engineering by genetic and combinatorial strategies. She has about 30 papers on these and related topics.
PUBLICATIONS
1. Liu Y.; Zheng H.; Qin W.; Zhan G.; Tian L; Li W. “Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain.” Sci. China Life Sci. 2014.
2. Li T.; Du Y.; Cui Q.; Zhang J.; Zhu W.; Hong K.; Li W. “Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.” Mar. Drugs 2013, 11, 466-488.
3. Gao M.; Song X.; Feng Y.; Li W.; Cui Q. “Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116.” J Oleo Sci. 2013, 62(3), 143-151.
4. Cui G.; Hong W.; Zhang J.; Li W.; Feng Y.; Liu C.; Cui Q. “Targeted gene engineering in Clostridium cellulolyticum H10 without methylation.” J. Microbiol. Methods. 2012, 89, 201-208.
5. Yang D.; Li W.; Huang S.; Shen B. “Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes." Org. Lett. 2012, 14(5), 1302-1305.
6. Luo Y.; Li W.; Ju J.; Yuan Q.; Peter N.R.; Hoffmann F.M.; Huang S.; Bugni T.S.; Rajski S.; Osada H.; Shen B. “Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis.” J. Am. Chem. Soc. 2010, 132, 6663-6671.
7. Ju J. *; Li W.*; Yuan Q.; Peters N.R.; Hoffmann F.M.; Rajski S.R.; Shen B. "Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity." Org. Lett. 2009, 11(7), 1639-1642. (* Contributed equally)
8. Li W.; Luo Y.; Ju J.; Rajski S.R.; Osada H.; Shen B. "Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes." J. Nat. Prod. 2009, 72, 450-459.
9. Lin S.; Geoffrey P.H.; Chen Y.; Li, W; Shen, B. "Characterization of the SgcF epoxide hydrolase supporting an (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis.” J. Am. Chem. Soc. 2009, 131, 16410-16417.
10. Pinchot, S.N.; Adler, J.T.; Luo, Y.; Ju, J.; Li, W; Shen, B; Kunnimalaiyaan, M.; Chen, H. "Tautomycin suppresses growth and neuroendocrine hormone markers in carcinoid cells through activation of the Raf-1 pathway. " Am. J. Surg. 2009, 197(3), 313-319.
11. Li W., Ju J., Osada H., Shen B. " Characterization of the Tautomycin Biosynthetic Gene Cluster from Streptomyces spiroverticillatus Unveiling New Insights into Dialkylmaleic Anhydride and Polyketide Biosynthesis." J. Bio. Chem. 2008, 283(42), 28607-28617.
12. Zhang J.; Van Lanen S. G.; Ju J.; Liu W.; Dorrestein P.C.; Li W.; Kelleher N.L.; Shen B. "A self-phosphopantetheinylating polyketide synthase producing a linear polyene to initiate the biosynthesis of the enediyne antitumor antibiotics." PNAS. 2008, 105(5), 1460-1465.
13. Xie Z*.; Li W*.; Tian Y.; Liu G.; Tan H. "Identification and characterization of sawC, a whiA-like gene, essential for sporulation in Streptomyces ansochromogenes." Arch. Microbiol. 2007, 188, 575-582. (* Contributed equally)
14. Li W.; Ju J.; Osada H.; Shen B. "Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus." J. Bacteriol. 2006, 188(11), 4148-4152.
15. Dorrestein P.C.; Van Lanen S.G.; Li W.; Zhao C.; Deng Z.; Shen B.; Kelleher N.L. "The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis." J. Am. Chem. Soc. 2006, 128(32), 10386-10387.
16. Li Y.; Ling H.; Li W.; Tan H. "Improvement of nikkomycin production by enhanced copy of sanU and sanV in Streptomyces ansochromogenes and characterization of a novel glutamate mutase encoded by sanU and sanV." Metab. Eng. 2005, 7(3), 165-173.
17. Li W.; Tan H. "Structure and function of sanV: a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes." Curr. Microbiol. 2003, 46(6), 403-407.
18. Li W.; Liu G.; Tan H. "Disruption of sabR affects nikkomycin biosynthesis and morphogenesis in Streptomyces ansochromogenes." Biotechnol. Lett. 2003, 25, 1491-1497.
19. Li W.; Jia J.; Tan H. "Cloning, sequencing and function of sanB, a gene related to nikkomycin biosynthesis of Streptomyces ansochromogenes." Chin. Sci. Bull. 2000, 45(23), 2158-2162.
20. Jia J.; Li W.; Tan H. "Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes." Sci. in China, Ser. C, 2000, 43(1), 30-38. 15. Nie L.; Li W.; Tan H. "Cloning and function of sanK-a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes." Chin. J. Biotech., 2000, 16(6), 667-670.
