FengHe
Lecturer, Laboratory of fish physiology and reproductive
Department of Aquaculture, Fisheries College, Ocean University of China
5 Yushan Road, Phone: 86-532-82031953
Qingdao 266003, Fax: 86-532-82031953
P. R. China Email:hefengouc@ouc.edu.cn
EDUCATION
Ph.D. in Animal genetic breed and reproduction Science, degree awarded in July 2006, China Agricultural University, Beijing city, China
M.S. in Animal genetic breed and reproduction Science, degree awarded in July 2003, Jilin Agricultural University, Changchun city, China
B.A. in Animal Science, degree awarded in July 2000, Yangzhou University, Yangzhou city, China
RESEARCH
Research Interests:
My research focuses on mechanisms and controls of fish growth and reproductive. Our research has an emphasis on Single-nucleotide polymorphism (SNP) and DNA methylation. One-basevariation including deletion, insertion, and substitution,plays an important role in the transcription andtranslation of genes and affects function of protein. DNA methylation constitutes an important epigenetic factor in the control of genetic information. Gene expression is controlled not only by the presence of regulatory sequences, but also by the chromatin configuration and the DNA methylation status of the specific genes. These findings may help us toelucidate the molecular mechanism of endocrine physiology in fish from the epigenetic point of view.
Specific Research Directions:
1. SNP in the candidate genes related to growth and reproductive. Single nucleotide polymorphisms (SNP), one base variant includingdeletion, insertion, and substitution, can greatly influence geneexpression and the functions of proteins. In agricultural and aquaculturespecies, SNPs are especially important if they cause differencesin economic traits, or are linked to the mutations that do so. We have optimized the SSCPprocedure to detect single nucleotide polymorphisms (SNPs) and usedthis method to evaluate polymorphisms of growth and reproductive genes and theirassociations with growth and reproductive traits.
2.DNA methylation regulating mRNA expression pattern of growth and reproductive genes during different growth and reproductive development stages. DNA methylation emerged as an important epigenetic modification by regulating gene expression. In general, DNA methylation represses transcription, and loss of methylation is associated with gene activation. In addition, recent evidence indicated that DNA methylation of the coding region was more powerful in suppressing gene expression than that of the promoter region. Hence, in order to elucidate the mechanism behind the regulation of functional candidate genes expression in different ovarian development stage and growth development of fish. So it suggested that methylation at these sites might account for the decreased expression of those genes.
Current Research Projects (recent 10 years):
§ The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China (KLM201201)
§ Natural Science Foundation of Shandong Province, China (No. ZR2009DQ011)
§ Research Fund for the Doctoral Program of Higher Education of China (20090132120006)
§ The open-fund of Key Laboratory of Fisheries Genetic Resources &Aquaculture, Chinese Academy of Fisheries Sciences (2008B1207).
§ Postdoctoral Innovative Projects of Shandong Province, China (No. 200702039).
§ State 863 High- Technology R&D Project of China (2012AA10A403)
PUBLICATION (recent 10 years)
（* corresponding author）
Feng He, HaiShen Wen*, ShuangLin Dong, Bao Shi, CaiFang Chen, LianShun Wang, Jun Yao, XingJiang Mu, YuGuo Zhou. Identification of single nucleotide polymorphism cytochrome P450-c19a and its relation to reproductive traits in Japanese flounder (Paralichthys olivaceus). Aquaculture, 2008, 279:177-181.
Feng He, HaiShen Wen*, ShuangLin Dong, LianShun Wang, CaiFang Chen, Bao Shi, XingJiang Mu, Jun Yao, YuGuo Zhou. Identification of estrogen receptor α gene polymorphisms by SSCP and its effect on reproductive traits in Japanese flounder (Paralichthys olivaceus). Comparative Physiology and Biochemistry Part B. 2008, 150: 278- 283.
Feng He, HaiShen Wen*, ShuangLin Dong, Bao Shi, CaiFang Chen , LianShun Wang, Jun Yao, XingJiang Mu, YuGuo Zhou. Polymorphisms within promoter of Japanese flounder (Paralichthys olivaceus) ovary cytochrome P450-c19(CYP19a) gene associated with reproductive traits. Fish Physiology and Biochemistry. 2009,35:333-340.
Bao Shi, HaiShen Wen*, Feng He, ShuangLin Dong, Shen Ma, CaiFang Chen, XiaoYan Chen, JiaRen, Zhang, GuoXiong Jin. Single nucleotide polymorphisms within the estrogen receptor beta gene are linked with reproductive indices in Japanese flounder, Paralichthys olivaceus. Comparative Physiology and Biochemistry Part B, 2009, 154:62-67.
Bao Shi, HaiShen Wen*, Feng He, ShuangLin Dong, Shen Ma, CaiFang Chen, LianShun Wang, Jun Yao, XingJiang Mu, YuGuo Zhou. Association of reproductive performance with SNPs of FOXL2 gene by SSCP in Japanese flounder (Paralichthys olivaceus).Comparative Physiology and Biochemistry Part B, 2009, 153:1-7.
Feng He *, HaiShen Wen, JiFang Li, DaHui Yu, RuiQin Ma, Dan Shi, YuanQing Zhang, Jian Hu,WeiJie Mu, Miao Liu, JiaNan Zhang, QingQing Wang, YuRen Yuan, Qun Liu. Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Performance of Japanese flounder (Paralichthys olivaceus). Asian-Australian journal of Animal Science. 2011, 24(4): 463-470.
RuiQin Ma, Feng He*, HaiShen Wen, JiFang Li, Bao Shi, Dan Shi, Miao Liu, WeiJie Mu, YuanQing Zhang, Jian Hu, WeiGuo Han, JiaNan Zhang, QingQing Wang, YuRen Yuan, and Qun Liu. Mutations in exons of the CYP17-II gene affect sex steroid concentration in male Japanese flounder (Paralichthys olivaceus). Journal Ocean University of China. 2012, 11 (1): 99-104.
RuiQin Ma, Feng He*, HaiShen Wen, JiFang Li, WeiJie Mu, Miao Liu, YuanQing Zhang, Jian Hu, Qun Liu. Polymorphysims of CYP17-ⅠGene in the Exons were Associated with the Reproductive Endocrine of Japanese founder (Paralichthys olivaceus). Asian- Australian journal of Animal Science. 2012, 25(6) :794 799 .
YuXia Ding , Feng He*, HaiShen Wen, JiFang Li, Kun Qian, MeiLi Chi, Meng Ni,XiangHan Yin, Yan Bu, YiJie Zhao, DongQian Zhang. Polymorphism in exons CpG rich regions of the cyp17-II gene affecting its mRNA expression and reproductive endocrine levels in female Japanese flounder (Paralichthys olivaceus). General and Comparative Endocrinology, 2012,179:107-114.
YuXia Ding, Feng He*, HaiShen Wen, JiFang Li, Meng Ni, MeiLi Chi, Kun Qian, Yan Bu, DongQian Zhang, YuFeng Si, JunLi Zhao. DNA methylation status of cyp17-Ⅱ gene correlatedwith its expression pattern and reproductive endocrinology during ovarian development stages of Japanese flounder (Paralichthys olivaceus).Gene, 2013, 527: 82-88.
